Coding

Part:BBa_K5210001:Design

Designed by: yitian zhou   Group: iGEM24_NMU-China   (2024-09-23)


LuxA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our luminescence circuit, LuxA is controlled by PumuDC, which can be activated by UV-C radiation. At night, LuxA and luxB express simultaneously, so LuxA can bind with luxB and form luxAB. LuxAB is a luciferase which can utilize the aldehyde made by LuxCDE to produce blue-green light. Experimental results about our butyrate-sensing circuit can be found in our composite part BBa xxx


Source

Yes

References